Terms with the suffix cide or cidal for killing action also are commonly used. Decontamination removes pathogenic microorganisms from objects so they are safe to handle, use, or discard. Thorough cleaning is essential before high-level disinfection and sterilization because inorganic and organic materials that remain on the surfaces of instruments interfere with the effectiveness of these processes. Germicides differ markedly, primarily in their antimicrobial spectrum and rapidity of action.Ĭleaning is the removal of visible soil (e.g., organic and inorganic material) from objects and surfaces and normally is accomplished manually or mechanically using water with detergents or enzymatic products. Intermediate-level disinfectants might be cidal for mycobacteria, vegetative bacteria, most viruses, and most fungi but do not necessarily kill bacterial spores.

Low-level disinfectants can kill most vegetative bacteria, some fungi, and some viruses in a practical period of time (≤10 minutes). At similar concentrations but with shorter exposure periods (e.g., 20 minutes for 2% glutaraldehyde), these same disinfectants will kill all microorganisms except large numbers of bacterial spores they are called high-level disinfectants. A few disinfectants will kill spores with prolonged exposure times (3–12 hours) these are called chemical sterilants. Unlike sterilization, disinfection is not sporicidal.

Each of the various factors that affect the efficacy of disinfection can nullify or limit the efficacy of the process.įactors that affect the efficacy of both disinfection and sterilization include prior cleaning of the object organic and inorganic load present type and level of microbial contamination concentration of and exposure time to the germicide physical nature of the object (e.g., crevices, hinges, and lumens) presence of biofilms temperature and pH of the disinfection process and in some cases, relative humidity of the sterilization process (e.g., ethylene oxide). In health-care settings, objects usually are disinfected by liquid chemicals or wet pasteurization. These same germicides used for shorter exposure periods also can be part of the disinfection process (i.e., high-level disinfection).ĭisinfection describes a process that eliminates many or all pathogenic microorganisms, except bacterial spores, on inanimate objects (Tables 1 and 2). Sterilization is intended to convey an absolute meaning unfortunately, however, some health professionals and the technical and commercial literature refer to “disinfection” as “sterilization” and items as “partially sterile.” When chemicals are used to destroy all forms of microbiologic life, they can be called chemical sterilants. Steam under pressure, dry heat, EtO gas, hydrogen peroxide gas plasma, and liquid chemicals are the principal sterilizing agents used in health-care facilities. Sterilization describes a process that destroys or eliminates all forms of microbial life and is carried out in health-care facilities by physical or chemical methods. 6-12 This guideline presents a pragmatic approach to the judicious selection and proper use of disinfection and sterilization processes the approach is based on well-designed studies assessing the efficacy (through laboratory investigations) and effectiveness (through clinical studies) of disinfection and sterilization procedures. 3-6 Failure to comply with scientifically-based guidelines has led to numerous outbreaks. Multiple studies in many countries have documented lack of compliance with established guidelines for disinfection and sterilization. Because sterilization of all patient-care items is not necessary, health-care policies must identify, primarily on the basis of the items’ intended use, whether cleaning, disinfection, or sterilization is indicated. Failure to properly disinfect or sterilize equipment carries not only risk associated with breach of host barriers but also risk for person-to-person transmission (e.g., hepatitis B virus) and transmission of environmental pathogens (e.g., Pseudomonas aeruginosa).ĭisinfection and sterilization are essential for ensuring that medical and surgical instruments do not transmit infectious pathogens to patients. A major risk of all such procedures is the introduction of pathogens that can lead to infection.

2Each procedure involves contact by a medical device or surgical instrument with a patient’s sterile tissue or mucous membranes. In the United States, approximately 46.5 million surgical procedures and even more invasive medical procedures-including approximately 5 million gastrointestinal endoscopies-are performed each year.

Example of medical asepsis